Uses of Column Technology in Blood Transfusion

Microtubes in which columns of gel or microspheres are contained have gained widespread acceptance for routine serological testing. The use of columns for blood grouping, antibody screening and identification, and compatibility testing have all been well described. Columns have also been shown to perform well in the identification of antibody immunoglobulin subclasses and in the investigation of drug induced hemolytic anemia. The development of multi skilled laboratories has been facilitated by the introduction of these techniques and anxieties about loss of traditional skills have proved to be unfounded. The development of automation able to identify, handle and interpret large numbers of column tests has also led to their introduction into busy serological laboratories. New developments to increase the range of tests for which gel columns can be used include the use of columns containing specific monoclonal antibodies for the diagnosis of paroxysmal hemoglobinuria. The ability to easily and accurately identify irregular antibodies in patients serum associated with similarly accurate grouping and compatibility testing has led to improved patient care and management and a more efficient and effective delivery of transfusion practice.