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Interleukin-11 Gene Expression in Human Lymphoid Malignancy.

G Ishii1, K Harigaya1, Y W Qiang1

  • 1a Department of Pathology , Chiba University , Chiba , Japan.

Hematology (Amsterdam, Netherlands)
|July 15, 2016
PubMed
Summary

Interleukin-11 (IL-11) transcripts are present in some lymphoid malignancies, like follicular lymphoma, but not secreted as active protein. This suggests IL-11 gene expression may be altered during lymphoid cell neoplastic transformation.

Keywords:
Interleukin-11constitutive expressionhuman lymphoid malignancy

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Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • Interleukin-11 (IL-11) shares functions with IL-6 in hematopoiesis.
  • Limited data exists on IL-11 expression in lymphoid malignancies.

Purpose of the Study:

  • To investigate the expression of IL-11 in lymphoid cell lines and malignant lymphoma.
  • To explore the regulation of IL-11 transcript levels in lymphoid cells.

Main Methods:

  • Reverse transcriptase polymerase chain reaction (RT-PCR) to detect IL-11 transcripts.
  • Northern blotting for IL-11 expression analysis.
  • IL-11 bioassay and ELISA to assess protein secretion.

Main Results:

  • IL-11 transcripts detected in multiple lymphoid cell lines, notably high in follicular lymphoma (FL18).
  • IL-11 transcript levels in FL18 were constitutive and not upregulated by stimulation, unlike bone marrow stromal cells (KM102).
  • FL18 cells did not secrete biologically active IL-11; transcript stability was similar in FL18 and KM102.

Conclusions:

  • IL-11 transcripts can be found in neoplastic lymphoid cells, including malignant lymphoma.
  • Constitutive IL-11 expression in FL18 suggests potential alterations in gene regulation during lymphoid cell transformation.
  • Further research is needed to understand the role of IL-11 in lymphoid malignancies.