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Updated: Mar 17, 2026

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
Published on: February 21, 2025
Modified gene lacZ encoding β-galactosidase as efficient transcriptional reporter for the Yarrowia lipolytica yeast
E V Trubnikova1, E P Isakova2, Yu I Deryabina3
1Kursk State University, Kursk, Russia.
Abstract:
A modified variant of the β-galactosidase gene from Escherichia coli (lacZ) has been obtained. This was designed for building transcriptional reporter constructs, the product of which possesses a higher proteolytic resistance in the Yarrowia lipolytica yeasts. The study of the activity of mitochondrial voltagedependent porin (voltage-dependent anion channel, VDAC) showed a high efficiency of the reporter system based on the designed lacZ reporter compared to the wild-type lacZ gene.
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