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Related Experiment Video

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Tools and Techniques for Wt1-Based Lineage Tracing.

Bettina Wilm1, Ramon Muñoz-Chapuli2

  • 1Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK. b.wilm@liv.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|July 16, 2016
PubMed
Summary
This summary is machine-generated.

This chapter reviews animal models for Wt1-expressing cell lineage analysis. It details genetic lineage tracing approaches and provides protocols for studying Wt1-derived cells in embryos.

Keywords:
Cell lineage tracingCre-ERT2Cre/loxPInducible Cre recombinaseRosa26 reporter miceTamoxifenWt1

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • The Wilms Tumor 1 (Wt1) gene plays a crucial role in development.
  • Understanding Wt1-expressing cell lineages is vital for developmental biology research.
  • Existing methods for lineage tracing have limitations.

Purpose of the Study:

  • To review and consolidate available animal models for Wt1-expressing cell lineage analysis.
  • To describe genetic lineage tracing strategies utilizing the Cre/loxP system under Wt1 promoter control.
  • To provide practical laboratory protocols for Wt1 lineage tracing studies.

Main Methods:

  • Review of existing literature on Wt1 lineage tracing models.
  • Description of constitutive and inducible Cre/loxP systems driven by Wt1 promoter.
  • Detailed protocols for tissue dissection, immunofluorescence, and histopathological analysis.

Main Results:

  • Identification and categorization of various Wt1 lineage tracing models.
  • Explanation of how Wt1 promoter drives Cre recombinase for lineage labeling.
  • Demonstration of methods for analyzing lineage-labeled Wt1-derived cells in embryonic tissues.

Conclusions:

  • A comprehensive overview of Wt1 lineage tracing models is presented.
  • Genetic tools based on the Cre/loxP system offer robust Wt1 cell lineage analysis.
  • The provided protocols facilitate experimental investigation of Wt1 function in development.