Related Experiment Video
Updated: Mar 17, 2026
09:21
Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
Published on: December 27, 2014
24.9K
Isolation and Colony Formation of Murine Bone and Bone Marrow Cells
Sophie McHaffie1, You-Ying Chau2,3
1MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, EH4 2XU, UK.
Methods in Molecular Biology (Clifton, N.J.)
|July 16, 2016
Abstract:
Adult homeostasis is dependent on normal Wt1 expression. Loss of Wt1 expression in adult mice causes rapid loss of the mesenchymal tissues, fat and bone, amongst other phenotypes. Bone and bone marrow mesenchymal stromal cells can be studied by cell isolation and expansion. The stemness of these cells can then be characterized by carrying out a colony-forming unit-fibroblast assay and observing clonogenic capabilities.
