Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.5K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.1K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.1K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.8K
3.8K
Transcription01:10

Transcription

158.4K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
158.4K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

26.8K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.8K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

4.2K
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Organized peripheral vascular strand development in nodules is controlled by a bHLH/HLH heterodimer.

The New phytologist·2026
Same author

A cytokinin-auxin antagonistic module participates in nitrogen-triggered tiller outgrowth in rice.

Plant physiology·2026
Same author

Procrustean pseudo-landmark methods in Python to measure massive quantities of leaf shape data.

Applications in plant sciences·2026
Same author

The genomic basis of adaptive leaf variation in the Galápagos giant daisies.

Nature communications·2026
Same author

LEAFY demonstrates functions in reproductive development of the gametophyte but not the sporophyte of the fern Ceratopteris richardii.

Development (Cambridge, England)·2026
Same author

LEAFY demonstrates functions in reproductive development of the gametophyte but not the sporophyte of the fern Ceratopteris richardii.

Development (Cambridge, England)·2025

Related Experiment Video

Updated: Mar 17, 2026

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

13.1K

eQTL Regulating Transcript Levels Associated with Diverse Biological Processes in Tomato.

Aashish Ranjan1, Jessica M Budke1, Steven D Rowland1

  • 1Department of Plant Biology, University of California, Davis, California 95616.

Plant Physiology
|July 16, 2016
PubMed
Summary
This summary is machine-generated.

This study mapped expression quantitative trait loci (eQTL) in tomato, revealing genetic hotspots that control gene expression patterns influencing plant traits like leaf development and photosynthesis.

More Related Videos

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

12.8K
Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
11:33

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

2.2K

Related Experiment Videos

Last Updated: Mar 17, 2026

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

13.1K
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

12.8K
Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
11:33

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

2.2K

Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Gene expression variation significantly impacts plant traits, alongside genetic sequence variations.
  • Expression quantitative trait loci (eQTL) are crucial for understanding the genetic basis of gene expression patterns.

Purpose of the Study:

  • To identify the genetic underpinnings of transcript level variation in tomato.
  • To characterize the impact of introgression from Solanum pennellii on the Solanum lycopersicum transcriptome.

Main Methods:

  • Utilized an introgression population derived from Solanum pennellii and Solanum lycopersicum.
  • Employed Barnes-Hut t-distributed stochastic neighbor embedding for clustering transcriptomic data.
  • Performed comprehensive eQTL analysis to identify genetic determinants of gene expression.

Main Results:

  • Identified approximately 7,200 eQTL regulating 5,300 genes.
  • Discovered 42 gene expression modules linking transcript levels to biological processes.
  • Uncovered genetic hotspots influencing plant defense and photosynthesis, and identified eQTL associated with leaf development and hypocotyl length.

Conclusions:

  • The genetic architecture of global transcript abundance in tomato is complex.
  • eQTL significantly influence plant phenotypes, with identified loci impacting leaf development and photosynthesis.
  • This research provides a valuable resource for studying eQTL effects on tomato traits.