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Related Concept Videos

Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

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Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Interactions Between Signaling Pathways01:19

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Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
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The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
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Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
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Related Experiment Video

Updated: Mar 17, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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The Hippo pathway, p53 and cholesterol.

Yael Aylon1, Moshe Oren1

  • 1a Department of Molecular Cell Biology , The Weizmann Institute of Science , Rehovot , Israel.

Cell Cycle (Georgetown, Tex.)
|July 16, 2016
PubMed
Summary

Cancer cells rewire metabolism, increasing cholesterol and lipid synthesis. The Hippo and p53 pathways cooperate to regulate sterol regulatory element-binding proteins (SREBPs), offering new targets for cancer intervention.

Keywords:
LATSRNF20SREBPYAPbistabilitymutant p53statins

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Related Experiment Videos

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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
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Area of Science:

  • Oncology
  • Molecular Biology
  • Metabolic Research

Background:

  • Cancer cells exhibit metabolic reprogramming, characterized by elevated cholesterol and lipid synthesis.
  • Sterol regulatory element-binding proteins (SREBPs) control genes vital for cholesterol and fatty acid biosynthesis.
  • SREBP activity is modulated by oncogenes and tumor suppressors, highlighting its role in cancer progression.

Purpose of the Study:

  • To investigate the interplay between the Hippo pathway, p53, and SREBP activity in cancer.
  • To elucidate how these pathways collectively regulate cholesterol and lipid metabolism in the context of tumorigenesis.
  • To identify potential novel therapeutic targets within the Hippo-p53-SREBP regulatory network.

Main Methods:

  • The study focuses on the regulatory interactions between the Hippo pathway, p53, and SREBPs.
  • Analysis of how these pathways influence the transcription of genes involved in cholesterol and lipid biosynthesis.
  • Conceptual exploration of therapeutic interventions targeting this network.

Main Results:

  • The Hippo and p53 signaling pathways cooperate to fine-tune SREBP activity.
  • This cooperation is crucial for regulating cholesterol and lipid levels in both normal and cancerous cells.
  • Dysregulation of this network contributes to the metabolic rewiring observed in cancer.

Conclusions:

  • The Hippo-p53-SREBP network represents a critical nexus for metabolic control in cancer.
  • While statins targeting cholesterol biosynthesis have limitations, this complex regulatory network offers promising avenues for novel cancer therapies.
  • Targeting the intricate regulation of lipid metabolism presents a potential strategy for future cancer interventions.