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Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
Published on: June 10, 2018
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The quest for improved reproducibility in MALDI mass spectrometry
Matthew B O'Rourke1, Steven P Djordjevic2, Matthew P Padula1
1Proteomics Core Facility, University of Technology Sydney, Cnr Harris and Thomas St, Ultimo, New South Wales, 2007, Australia.
Mass Spectrometry Reviews
|July 16, 2016
Summary
Improving reproducibility in MALDI mass spectrometry (MS) is crucial for quantitative analysis. Nitrocellulose substrates show promise for creating homogeneous crystal formation, enhancing the reliability of MALDI MS protocols.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
Background:
- Reproducibility remains a significant challenge in developing quantitative protocols for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI MS).
- The heterogeneous nature of sample recrystallization in MALDI MS often necessitates manual intervention for automated sample acquisition, impacting data consistency.
Purpose of the Study:
- To review the literature and anecdotal evidence from the past 30 years addressing reproducibility in MALDI MS.
- To identify promising strategies for developing universal preparation protocols for quantitative MALDI MS.
Main Methods:
- Comprehensive literature review of methods aimed at improving MALDI MS reproducibility.
- Analysis of anecdotal evidence regarding sample preparation techniques.
Main Results:
- A substantial body of literature highlights the use of nitrocellulose as a substrate to enhance crystal formation homogeneity.
- Nitrocellulose-based methods demonstrate potential for improving reproducibility in MALDI MS.
Conclusions:
- Nitrocellulose emerges as a highly promising substrate for achieving homogeneous crystal formation in MALDI MS.
- Further research into nitrocellulose-based protocols could lead to a comprehensive and universal method for quantitative MALDI MS analysis.
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