Gelling of urea-linked gelatin with fresh frozen plasma

Urea-linked gelatin and succinylated gelatin were mixed with fresh frozen plasma and human albumin solutions in vitro, in varying concentrations and at different temperatures. Gelling was only seen in those mixtures that contained fresh frozen plasma and urea-linked gelatin. It occurred at both room temperature and 37 degrees C when the mixture contained more than 40% by volume of urea-linked gelatin. Further tests confirmed that this was the result of calcium ions in the urea-linked gelatin allowing coagulation to occur in the fresh frozen plasma.