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Correlation between hepatitis B virus DNA levels and diagnostic tests for HBsAg, HBeAg, and PreS1-Ag in chronic

X Liu1, J M Chen1, J L Lou1

  • 1Clinical Laboratory Center, Beijing You'an Hospital, Capital Medical University, Beijing, China.

Genetics and Molecular Research : GMR
|July 16, 2016
PubMed

Insights

Serum HBsAg is a poor predictor of hepatitis B virus (HBV) replication. Co-detection of HBeAg and PreS1-Ag offers a more reliable method for diagnosing and managing chronic hepatitis B (CHB) patients.

Area of Science:

  • Hepatology
  • Virology
  • Clinical Diagnostics

Background:

  • Chronic hepatitis B (CHB) affects millions globally, necessitating accurate methods for monitoring viral activity.
  • Hepatitis B virus (HBV) DNA levels are crucial for assessing viral replication and guiding treatment decisions.
  • Current diagnostic markers like HBsAg and HBeAg have limitations in precisely quantifying HBV DNA.

Purpose of the Study:

  • To evaluate the diagnostic value of serum HBsAg, HBeAg, and PreS1-Ag in quantifying HBV DNA levels in CHB patients.
  • To determine the correlation between these serum markers and HBV DNA replication.
  • To identify optimal markers for predicting HBV DNA levels in CHB.

Main Methods:

  • Recruitment of 1000 CHB patients.
  • Detection of serum HBsAg and HBeAg using electrochemiluminescence immunoassay.
  • Quantification of PreS1-Ag by ELISA and HBV DNA by fluorescence quantitative PCR.

Main Results:

  • A low correlation was observed between HBsAg and HBV DNA (r = 0.172), which strengthened at high HBV DNA levels (log10 > 7, r = 0.597).
  • HBsAg and HBV DNA showed a significant positive correlation in HBeAg-positive patients (r = 0.321) but not in HBeAg-negative patients (r = -0.016).
  • HBV DNA expression correlated significantly with HBeAg (χ2 = 83.07) and PreS1-Ag (χ2 = 36.01). The HBV DNA-positive rate was highest in patients positive for both HBeAg and PreS1-Ag (72.26%).

Conclusions:

  • Serum HBsAg is not a reliable marker for predicting HBV replication.
  • Co-detection of HBeAg and PreS1-Ag provides a more accurate prediction of HBV DNA replication.
  • Combined HBeAg and PreS1-Ag detection is a valuable method for clinical diagnosis and treatment management of CHB.

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