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Published on: March 12, 2021
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Adipose Gene Expression Profile Changes With Lung Allograft Reperfusion
Joshua M Diamond1, Selim Arcasoy2, Jamiela A McDonnough2
1Pulmonary, Allergy, and Critical Care Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
Summary
Lung reperfusion alters gene expression in thoracic visceral adipose tissue (VAT). This study found significant changes in complement, coagulation, and Jak-STAT pathways, highlighting VAT
Area of Science:
- Immunology and Transplantation
- Molecular Biology and Genomics
- Metabolic Research
Background:
- Obesity is a known risk factor for primary graft dysfunction (PGD) after lung transplantation.
- The effects of ischemia-reperfusion injury on adipose tissue following lung transplantation remain largely uncharacterized.
- Understanding adipose tissue's response to transplantation is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the impact of lung allograft ischemia-reperfusion on gene expression within thoracic visceral adipose tissue (VAT).
- To identify specific molecular pathways affected by reperfusion in VAT.
- To explore potential links between adipose tissue gene expression and primary graft dysfunction.
Main Methods:
- Thoracic visceral adipose tissue (VAT) samples were collected from six lung transplant recipients before and after allograft reperfusion.
- Total RNA was isolated from VAT and gene expression was quantified using the Human Gene 2.0 ST array.
- Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed to identify enriched biological pathways.
Main Results:
- Ischemia-reperfusion significantly altered gene expression in thoracic VAT, with 72 genes upregulated and 56 downregulated.
- Enriched pathways included complement and coagulation cascades, and Janus kinase-signal transducer and activator of transcription (Jak-STAT) signaling.
- Long pentraxin-3 (a PGD-associated gene) was the most upregulated gene, while Fibronectin leucine-rich transmembrane protein-3 was most downregulated.
Conclusions:
- Ischemia-reperfusion demonstrably impacts gene expression in visceral adipose tissue in lung transplant recipients.
- The observed changes in gene expression suggest a role for VAT in the inflammatory response post-transplant.
- Further research is warranted to explore the relationship between differential adipose tissue gene expression and PGD development.
