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Updated: Mar 17, 2026
Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis
Published on: May 9, 2025
A rat model of oral submucous fibrosis induced by bleomycin
Shan-Shan Zhang1, Zhao-Jian Gong2, Wei Xiong3
1Department of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Objective:
The aim of this study was to induce a rat model of oral submucous fibrosis (OSF) using bleomycin.
Study Design:
A randomized trial was performed. Bleomycin dilution or phosphate-buffered saline was injected daily into the buccal mucosa of the test and control rats, respectively, for 2, 4, 6, or 8 weeks. Changes in histopathologic features, myofibroblasts, ultrastructure, and the levels of collagen type I and III, transforming growth factor-beta 1, and interferon-γ were measured.
Results:
Compared with controls, the buccal mucosa of the test rats appeared pale and stiff. There was collagen deposition in the laminae propria. Type I and type III collagen increased steadily, transforming growth factor-beta 1 increased and then decreased, and interferon-γ was gradually reduced. The ultrastructure was also similar to that of OSF.
Conclusions:
Using bleomycin, we induced a rat model of OSF that is very similar to human OSF in clinical manifestations, pathology, and ultrastructural changes.
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