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Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
Methane production from formate, acetate and H2/CO2; focusing on kinetics and microbial characterization
Xiaofang Pan1, Irini Angelidaki2, Merlin Alvarado-Morales2
1Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China; Department of Environment Engineering, Technical University of Denmark, Copenhagen Lyngby 2800, Denmark.
Abstract:
For evaluating the methanogenesis from typical methanogenic precursors (formate, acetate and H2/CO2), CH4 production kinetics were investigated at 37±1°C in batch anaerobic digestion tests and stimulated by modified Gompertz model. The results showed that maximum methanation rate from formate, acetate and H2/CO2 were 19.58±0.49, 42.65±1.17 and 314.64±3.58NmL/gVS/d in digested manure system and 6.53±0.31, 132.04±3.96 and 640.16±19.92NmL/gVS/d in sewage sludge system during second generation incubation. Meanwhile the model could not fit well in granular sludge system, while the rate of formate methanation was faster than from H2/CO2 and acetate. Considering both the kinetic results and microbial assay we could conclude that H2/CO2 methanation was the fastest methanogenic step in digested manure and sewage sludge system with Methanomicrobiales as dominant methanogens, while granular sludge with Methanobacteriales as dominant methanogens contributed to the fastest formate methanation.
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