Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sludge biolysis pretreatment to reduce antibiotic resistance genes (ARGs): Insight into the relationship between potential ARGs hosts and BALOs' preferred prey.

Water research·2024
Same author

Evaluation of the passive mast cell activation test for identifying allergens in perioperative anaphylaxis: a study protocol for a prospective diagnostic accuracy study.

BMJ open·2024
Same author

Association between different triglyceride glucose index-related indicators and depression in premenopausal and postmenopausal women: NHANES, 2013-2016.

Journal of affective disorders·2024
Same author

Objective Noninvasive Measurement of the Volumizing Effect of a Dermal Filler: An In Vivo Study.

Aesthetic plastic surgery·2024
Same author

Quantitative proteomics reveals the dynamic proteome landscape of zebrafish embryos during the maternal-to-zygotic transition.

iScience·2024
Same author

Neural Stem Cell-Derived Small Extracellular Vesicles: key Players in Ischemic Stroke Therapy - A Comprehensive Literature Review.

International journal of nanomedicine·2024

Related Experiment Video

Updated: Mar 17, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

10.2K

TopPIC: a software tool for top-down mass spectrometry-based proteoform identification and characterization.

Qiang Kou1, Likun Xun1, Xiaowen Liu1,2

  • 1Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA.

Bioinformatics (Oxford, England)
|July 18, 2016
PubMed
Summary

Top-down mass spectrometry analyzes intact proteins, but interpreting the data is complex. TopPIC is a new tool that efficiently identifies and characterizes complex proteoforms with unknown alterations in high-throughput proteomics.

More Related Videos

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

9.3K
Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

1.2K

Related Experiment Videos

Last Updated: Mar 17, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

10.2K
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

9.3K
Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

1.2K

Area of Science:

  • Proteomics
  • Biochemistry
  • Computational Biology

Background:

  • Top-down mass spectrometry (MS) offers a comprehensive view of intact proteoforms, complementing bottom-up MS approaches.
  • Interpreting complex top-down tandem mass spectra presents a significant challenge in high-throughput proteomics.
  • Characterizing proteoforms with unknown primary structure alterations requires advanced analytical tools.

Purpose of the Study:

  • To develop and present TopPIC, a computational tool for efficient interpretation of top-down tandem mass spectra.
  • To enable the identification and characterization of complex proteoforms, including those with post-translational modifications and mutations.
  • To facilitate high-throughput proteome-level studies using top-down MS data.

Main Methods:

  • Development of the TopPIC software tool.
  • Utilizing a database search strategy for top-down tandem mass spectra.
  • Algorithm designed to identify and characterize proteoforms with unknown primary structure alterations.

Main Results:

  • TopPIC provides efficient identification and characterization of complex proteoforms.
  • The tool is capable of detecting unknown amino acid mutations and post-translational modifications.
  • Enables high-throughput analysis of top-down MS data for proteome-level studies.

Conclusions:

  • TopPIC significantly advances the analysis of top-down mass spectrometry data.
  • Facilitates a deeper understanding of proteoform diversity and alterations in biological systems.
  • Offers a valuable solution for complex proteoform characterization in proteomics research.