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Updated: Mar 17, 2026

Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry
Published on: November 21, 2025
H3K4 acetylation, H3K9 acetylation and H3K27 methylation in breast tumor molecular subtypes
Gaëlle Judes1,2, Aslihan Dagdemir1,2, Seher Karsli-Ceppioglu1,2,3
1Department of Oncogenetics, Centre Jean Perrin, CBRV, 28 Place Henri Dunant, 63001 Clermont-Ferrand, France.
Aim:
Here, we investigated how the St Gallen breast molecular subtypes displayed distinct histone H3 profiles.
Patients & Methods:
192 breast tumors divided into five St Gallen molecular subtypes (luminal A, luminal B HER2-, luminal B HER2+, HER2+ and basal-like) were evaluated for their histone H3 modifications on gene promoters.
Results:
ANOVA analysis allowed to identify specific H3 signatures according to three groups of genes: hormonal receptor genes (ERS1, ERS2, PGR), genes modifying histones (EZH2, P300, SRC3) and tumor suppressor gene (BRCA1). A similar profile inside high-risk cancers (luminal B [HER2+], HER2+ and basal-like) compared with low-risk cancers including luminal A and luminal B (HER2-) were demonstrated.
Conclusion:
The H3 modifications might contribute to clarify the differences between breast cancer subtypes.
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