Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases
Gary Yates1, Anjil Srivastava1, Beatriz Orosa1
1School of Biological and Biomedical Sciences, University of Durham, Durham, DH1 3LE, UK.
Methods in Molecular Biology (Clifton, N.J.)
|July 19, 2016
Abstract:
The conjugation of SUMO can profoundly change the behavior of substrate proteins, impacting a wide variety of cellular responses. SUMO proteases are emerging as key regulators of plant adaptation to its environment because of their instrumental role in the SUMO deconjugation process. Here, we describe how to express, purify, and determine SUMO deconjugation activity of a plant SUMO protease.
