Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Thermosensation01:43

Thermosensation

34.8K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
34.8K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

8.0K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
8.0K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

3.5K
3.5K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

4.6K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.6K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

6.9K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
6.9K
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

7.0K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Examining spatial variation and inequity in COVID-19 immunisation coverage in Aotearoa New Zealand: a nationwide geospatial study.

Vaccine·2026
Same author

Otitis media at 6-monthly assessments of Australian First Nations children between ages 12-36 months: Findings from two randomised controlled trials of combined pneumococcal conjugate vaccines.

International journal of pediatric otorhinolaryngology·2023
Same author

Examining spatial variation for immunisation coverage in pregnant women: A nationwide and geospatial retrospective cohort study in Aotearoa New Zealand.

Social science & medicine (1982)·2023
Same author

Key lessons from the COVID-19 public health response in Australia.

The Lancet regional health. Western Pacific·2022
Same author

Reply to letter: Retrospective cost-effectiveness of the 23-valent pneumococcal polysaccharide vaccination program in Australia.

Vaccine·2019
Same author

Pneumonia hospitalisation and case-fatality rates in older Australians with and without risk factors for pneumococcal disease: implications for vaccine policy.

Epidemiology and infection·2019

Related Experiment Video

Updated: Mar 17, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

10.7K

Modulation of TRPV4 by diverse mechanisms.

W G Darby1, M S Grace2, S Baratchi1

  • 1School of Health and Biomedical Sciences, RMIT University, Melbourne, Australia.

The International Journal of Biochemistry & Cell Biology
|July 19, 2016
PubMed
Summary

Transient receptor potential vanilloid 4 (TRPV4) channels are crucial for physiological functions. Understanding TRPV4 regulation is key to developing new therapies for various diseases.

Keywords:
Cell signallingIon channelMechanosensationOsmosensationPost-translational modificationReceptor signallingTRPV4Transient receptor potential

More Related Videos

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

15.8K
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

8.5K

Related Experiment Videos

Last Updated: Mar 17, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

10.7K
A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

15.8K
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

8.5K

Area of Science:

  • Molecular Biology
  • Physiology
  • Pharmacology

Background:

  • Transient receptor potential (TRP) channels are a diverse superfamily of ion channels activated by various stimuli.
  • TRP vanilloid 4 (TRPV4) channels respond to heat, mechanical stress, and osmotic changes, and are increasingly recognized for their modulation by intracellular signaling cascades.
  • While TRPV4 knockout mice exhibit mild phenotypes, specific mutations cause severe developmental issues, highlighting the channel's critical roles.

Purpose of the Study:

  • To review the signaling mechanisms that modulate TRPV4 function.
  • To explore the diverse physiological and pathophysiological roles of TRPV4 across multiple tissues.
  • To underscore the therapeutic potential of targeting TRPV4 regulation.

Main Methods:

  • Literature review of studies on TRPV4 channel function and regulation.
  • Analysis of research implicating TRPV4 in various physiological processes and disease states.
  • Synthesis of information on post-translational modifications and intracellular signaling pathways affecting TRPV4.

Main Results:

  • TRPV4 function is modulated by post-translational modifications and intracellular signaling, suggesting it acts as a signal transducer.
  • TRPV4 plays significant roles in pain sensation, cardiovascular function, barrier integrity, and respiratory system regulation.
  • Dysregulation of TRPV4 is implicated in conditions such as skeletal dysplasia, hypertension, diabetes, and respiratory diseases.

Conclusions:

  • Modulation of TRPV4 opening is a vital signaling component across numerous tissues.
  • Understanding TRPV4 regulation offers potential for novel therapeutic strategies for a wide range of diseases.
  • TRPV4's broad expression and involvement in critical pathways make it a promising therapeutic target.