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Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded
A Slama-Schwok1, M Rougée, V Ibanez
1Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, Paris, France.
Biochemistry
|April 18, 1989
Abstract:
The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.