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ATP does not regulate the reconstituted glucose transporter.

T J Wheeler1

  • 1Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292.

Biochemistry
|April 18, 1989
PubMed
Summary
This summary is machine-generated.

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Adenosine triphosphate (ATP) does not allosterically regulate glucose transport in human erythrocytes as previously hypothesized. This study found ATP primarily inhibits transport at millimolar concentrations, challenging existing models.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Membrane Transport

Background:

  • Previous research suggested adenosine triphosphate (ATP) influences glucose transport in human red blood cells (erythrocytes) and their vesicles.
  • Models proposed ATP allosterically or covalently regulates the glucose transporter's catalytic properties, with a proposed reduction in Km for uptake.
  • These interpretations were based on studies involving micromolar ATP levels and erythrocyte ghosts or purified transporters.

Purpose of the Study:

  • To investigate whether allosteric regulation of the glucose transporter by ATP occurs.
  • To test the proposed model of ATP-mediated regulation of glucose transport activity.

Main Methods:

  • Reconstituted glucose transport activity into liposomes using detergent-free erythrocyte membranes.

Related Experiment Videos

  • Examined the effects of ATP on transport when present inside, outside, or both sides of the liposomes.
  • Tested ATP effects on trypsin-treated liposomes to ensure transporter orientation specificity.

    • Main Results:

    • Observed only inhibition of glucose transport by ATP, contradicting the predicted activation.
    • Significant inhibition occurred only at millimolar ATP concentrations, not micromolar levels previously reported.
    • ATP's inhibitory effect was primarily on the extracellular surface, and the effects were nonspecific, mimicked by tripolyphosphate.

    Conclusions:

    • The results do not support a model of allosteric ATP regulation of the glucose transporter.
    • ATP's role in regulating glucose transport appears to be inhibitory at higher concentrations and non-specific.
    • Further research is needed to elucidate the precise mechanisms and physiological relevance of ATP's interaction with glucose transporters.