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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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Molecular architecture of the complete COG tethering complex
Jun Yong Ha1, Hui-Ting Chou2, Daniel Ungar1
1Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
Nature Structural & Molecular Biology
|July 19, 2016
Summary
The conserved oligomeric Golgi (COG) complex
Area of Science:
- Cell Biology
- Molecular Biology
- Structural Biology
Background:
- The conserved oligomeric Golgi (COG) complex is essential for vesicular trafficking within the Golgi apparatus.
- Understanding COG complex structure is key to elucidating its function in intracellular transport.
Purpose of the Study:
- To determine the three-dimensional architecture of the hetero-octameric COG complex from Saccharomyces cerevisiae.
- To provide structural insights into the mechanism of COG-mediated vesicle capture and fusion.
Main Methods:
- Negative-stain electron microscopy was employed to visualize the intact COG complex.
- Structural analysis was performed on the purified hetero-octameric complex from yeast.
Main Results:
- The intact COG complex exhibits an intricate, multi-legged structure.
- The architecture features four (or possibly five) flexible legs, suggesting adaptability in function.
- COG complex structure is distinct from that of the exocyst complex.
Conclusions:
- The unique architecture of the COG complex supports its role in vesicle capture and fusion.
- Structural elucidation provides a foundation for further functional studies of COG in Golgi trafficking.
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