Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

10.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The oxygen sensitivity of [4Fe-4S] clusters on the nitrogenase scaffold protein NifU.

Journal of inorganic biochemistry·2026
Same author

Trypsin digestion to prevent acid induced precipitation of metalloproteins for accurate ICP-MS analysis: Nitrogenase case study.

Analytical biochemistry·2025
Same author

Computationally Guided Design of a Soluble, Abundant and Functional AnfH Variant for the Expression of Fe-Only Nitrogenase in Plant Mitochondria.

Plant biotechnology journal·2025
Same author

De Novo Engineering of Solid-State Metalloproteins Using Recombinant Coiled-Coil Silk.

ACS biomaterials science & engineering·2021
Same author

Insights into Nitrogenase Bioelectrocatalysis for Green Ammonia Production.

ChemSusChem·2020
Same author

Engineering a solid-state metalloprotein hydrogen evolution catalyst.

Scientific reports·2020

Related Experiment Video

Updated: Mar 17, 2026

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

8.1K

Solid-State Metalloproteins-An Alternative to Immobilisation.

Trevor D Rapson1

  • 1Commonwealth Scientific and Industrial Research Organisation (CSIRO), Black Mountain, Canberra, ACT 2601, Australia. trevor.rapson@csiro.au.

Molecules (Basel, Switzerland)
|July 19, 2016
PubMed
Summary

Researchers engineered a novel silk protein material that incorporates metal active sites. This solid-state metalloprotein offers remarkable stability and controllable reactivity, presenting a new alternative to traditional immobilization methods.

Keywords:
biocatalysisbiosensorsde novo engineeringindustrial biotechnologysilk

More Related Videos

Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

19.0K
Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization
08:13

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

Published on: December 3, 2020

5.0K

Related Experiment Videos

Last Updated: Mar 17, 2026

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

8.1K
Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

19.0K
Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization
08:13

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

Published on: December 3, 2020

5.0K

Area of Science:

  • Biomaterials Science
  • Protein Engineering
  • Biochemistry

Background:

  • Traditional methods for immobilizing metalloproteins often face limitations in stability and control.
  • Developing robust and adaptable solid-state materials for catalytic applications is an ongoing challenge.

Purpose of the Study:

  • To introduce a novel protein engineering strategy for creating solid-state metalloprotein materials.
  • To demonstrate the stability and controlled reactivity of these engineered materials.

Main Methods:

  • Utilizing a recombinant silk protein scaffold for direct coordination of metal active sites.
  • Incorporating metal centers into the silk protein structure to form solid-state metalloprotein materials.

Main Results:

  • The engineered silk protein directly coordinates metal centers, mimicking natural metalloprotein reactivity.
  • The resulting solid-state materials exhibit exceptional stability across various temperatures and solvent conditions.

Conclusions:

  • Protein engineering offers a viable alternative to traditional immobilization for creating functional metalloprotein materials.
  • These stable, engineered metalloprotein materials have potential applications in diverse fields requiring robust biocatalysis.