Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

SDS-PAGE

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Microbiome-mediated pharmacology of ginseng: Mechanistic insights into metabolic regulation and therapeutic potential.

Journal of ginseng research·2026

Same author

Comparative Study of Redox Status of MDCK Cells in Chicken Embryo Extract Versus Fetal Bovine Serum.

International journal of molecular sciences·2026

Same author

Pharmacological evaluation of 1-acetyl-β-carboline, a naturally occurring compound with anti-skin cancer potential.

Molecular therapy. Oncology·2025

Same author

Microengineered patient-derived endometrium-on-a-chip for the evaluation of endometrial receptivity and personalised translational medicine.

Nature communications·2025

Same author

Modulating the BI-1(TMBIM6)/AKT Signaling Pathway by Licochalcone D Treatment Inhibits Human Melanoma Cell Growth.

Phytotherapy research : PTR·2025

Same author

Cancer-metastasis-on-a-chip reveals efficacy of bevacizumab and siRNA in overcoming carboplatin resistance in SKOV3 ovarian cancer within a fibrotic metastatic microenvironment.

Materials today. Bio·2025

Search research articles

Related Experiment Video

Updated: Mar 17, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Advanced negative detection method comparable to silver stain for SDS-PAGE separated proteins detection.

Xu Wang¹, Sun-Young Hwang², Wei-Tao Cong¹

¹School of Pharmacy, Wenzhou Medical College, Wenzhou, Zhejiang, China.

Analytical Biochemistry

|July 20, 2016

Summary

A new Phloxine B stain offers an easy, rapid, and sensitive method for detecting proteins in polyacrylamide gels, comparable to silver staining.

Keywords:

Background stain Negative stain Phloxine B Protein detection SDS-PAGE

More Related Videos

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Published on: August 14, 2009

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Related Experiment Videos

Last Updated: Mar 17, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Published on: August 14, 2009

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Area of Science:

Biochemistry
Proteomics
Analytical Chemistry

Background:

Protein detection in polyacrylamide gels is crucial for proteomics.
Existing methods like silver stain and imidazole-zinc stain have limitations in speed, sensitivity, or ease of use.

Purpose of the Study:

To develop an easy, rapid, and sensitive protein detection protocol for polyacrylamide gels.
To introduce Phloxine B as an advanced negative staining method.

Main Methods:

Proteins in polyacrylamide gels were incubated with Phloxine B.
Gels were subsequently developed in an acidic solution.
The method was compared to silver stain and imidazole-zinc stain for sensitivity and contrast.

Main Results:

Phloxine B staining allows for selective transparency of protein-dye complexes against an opaque background.
Detection of as little as 0.1-0.4 ng of gel-separated proteins is possible within 50 minutes.
Phloxine B staining demonstrated higher sensitivity and better contrast than imidazole-zinc stain.

Conclusions:

Phloxine B provides a sensitive and practical alternative for protein detection in polyacrylamide gels.
This method simplifies proteomics workflows by being rapid and requiring no specific observation time.
The technique offers a valuable tool for proteomics research.