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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
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Nestin-Based Reporter Transgenic Mouse Lines.

John Mignone1, Natalia Peunova1, Grigori Enikolopov2

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 20, 2016
PubMed
Summary
This summary is machine-generated.

New transgenic mouse lines utilize fluorescent proteins to identify and track neural stem and progenitor cells. These tools aid in studying stem cells across various tissue types, offering insights into cell development and regeneration.

Keywords:
Hair-follicle-associated pluripotent (HAP)ImmunocytochemistryNestinNestin-GFPReporter linesStem cellsTransgenic animals

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Area of Science:

  • Stem cell biology
  • Developmental biology
  • Genetics

Background:

  • Nestin is a key marker for neural stem and progenitor cells.
  • Existing transgenic models have limitations in broad tissue application.
  • Identifying and isolating diverse stem cell populations remains a challenge.

Purpose of the Study:

  • To generate novel transgenic mouse lines for visualizing nestin-expressing cells.
  • To demonstrate the utility of these lines in identifying and analyzing stem and progenitor cells across multiple tissue origins.
  • To provide a tool for broader stem cell research beyond the neural lineage.

Main Methods:

  • Generation of transgenic mouse lines with fluorescent protein (FP) expression under nestin gene regulatory control.
  • Utilizing these lines to identify, track, and isolate stem and progenitor cells.
  • Analysis of stem and progenitor cell populations in various adult tissues (ectodermal, endodermal, mesodermal).

Main Results:

  • Successful generation of transgenic mouse lines accurately reflecting nestin expression patterns.
  • Demonstrated ability to identify and visualize neural stem and progenitor cells.
  • Extended application shown for identifying stem and progenitor cells in non-neural tissues.

Conclusions:

  • The developed transgenic mouse lines are effective tools for identifying and analyzing nestin-expressing stem and progenitor cells.
  • These lines offer a versatile platform for studying stem cell biology across diverse tissue types.
  • This technology facilitates advancements in stem cell research and potential therapeutic applications.