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Discovery of HAP Stem Cells.

Lingna Li1, Robert M Hoffman2,3

  • 1AntiCancer Inc., 7917 Ostrow Street, San Diego, CA, 92111, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 20, 2016
PubMed
Summary
This summary is machine-generated.

Nestin-driven green fluorescent protein (ND-GFP) mice reveal neural and hair follicle stem cells express nestin. These nestin-expressing cells, termed hair-follicle-associated pluripotent (HAP) stem cells, are located in the hair follicle bulge and outer-root sheath.

Keywords:
BulgeGFPHair follicleNestin

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Area of Science:

  • Stem cell biology
  • Dermatology
  • Neuroscience

Background:

  • Nestin is a key marker for neural stem cells.
  • The hair follicle harbors stem cells crucial for hair regeneration.
  • Understanding stem cell markers can elucidate cell relationships and functions.

Purpose of the Study:

  • To visualize and characterize nestin-expressing cells in vivo using a transgenic mouse model.
  • To investigate the dynamic localization of nestin-positive cells within the hair follicle during the hair cycle.
  • To explore the potential relationship between neural stem cells and hair follicle stem cells.

Main Methods:

  • Generation of nestin-driven GFP (ND-GFP) transgenic mice.
  • Microscopic analysis of GFP expression in hair follicles during different hair cycle phases (anagen, telogen).
  • Identification and characterization of nestin-expressing cell morphology and location.

Main Results:

  • ND-GFP mice enabled selective labeling of neural and other stem cells, including those in the hair follicle.
  • Nestin-expressing cells were identified in the hair follicle bulge, below the sebaceous glands, during early anagen.
  • The location of these GFP-positive cells shifted from the bulge to the outer-root sheath during mid- and late-anagen, indicating dynamic behavior.
  • Nestin expression was observed in both neural and hair follicle stem cells, suggesting a shared characteristic or origin.

Conclusions:

  • Nestin is a reliable marker for identifying stem cells within the hair follicle bulge and outer-root sheath.
  • The dynamic localization of nestin-expressing cells correlates with hair follicle regeneration phases.
  • The shared expression of nestin in neural and hair follicle stem cells supports a potential relationship between these cell populations.
  • Nestin-expressing hair follicle stem cells were identified and termed hair-follicle-associated pluripotent (HAP) stem cells.