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Classical and atypical resistance of cancer cells as a target for resveratrol

Sylwia Borska1, Monika Pedziwiatr1, Monika Danielewicz1

  • 1Department of Histology and Embryology, Medical University, 50-368 Wroclaw, Poland.

Oncology Reports
|July 20, 2016
PubMed

Insights

Resveratrol shows promise in overcoming cancer cell resistance to chemotherapy by inhibiting proliferation and affecting key resistance proteins. This natural compound may help sensitize cancer cells to existing anticancer drugs.

Area of Science:

  • Oncology
  • Pharmacology
  • Biochemistry

Background:

  • Cancer cell resistance to chemotherapy is a major obstacle in effective anticancer therapy.
  • Identifying agents that can resensitize cancer cells to chemotherapeutics is a critical research area.
  • Resveratrol, a natural polyphenol, exhibits pleiotropic effects on cancer cells and is being investigated for its potential in overcoming drug resistance.

Purpose of the Study:

  • To evaluate the efficacy of resveratrol in inhibiting human pancreatic cancer cell proliferation.
  • To assess the impact of resveratrol on the levels of proteins associated with cytostatic drug resistance.
  • To investigate resveratrol's potential to overcome chemoresistance in specific pancreatic cancer cell lines.

Main Methods:

  • Utilized human pancreatic cancer cell lines with varying resistance profiles (EPP85-181P, EPP85-181RDB, EPP85-181PRNOV).
  • Assessed cell viability and proliferation using the Sulforhodamine B (SRB) assay.
  • Analyzed cell cycle arrest and protein expression (P-glycoprotein, Topoisomerase II α/β) via Flow Cytometry (FACS) and Western blot analysis, respectively.
  • Visualized protein localization using immunofluorescence techniques.

Main Results:

  • Resveratrol demonstrated significant inhibition of proliferation across all tested pancreatic cancer cell lines.
  • Resveratrol reduced the expression and activity of P-glycoprotein (P-gp) in daunorubicin-resistant cells (EPP85-181RDB).
  • A statistically significant increase in both isoforms of Topoisomerase II (TopoII) was observed in mitoxantrone-resistant cells (EPP85-181PRNOV) upon resveratrol treatment.

Conclusions:

  • Resveratrol effectively inhibits pancreatic cancer cell proliferation in vitro.
  • Resveratrol modulates key proteins involved in chemoresistance, including P-glycoprotein and Topoisomerase II.
  • These findings suggest that resveratrol holds potential as an adjuvant therapy to overcome cancer cell resistance to chemotherapeutic drugs.

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