Related Experiment Video
Updated: Mar 17, 2026

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
Published on: May 16, 2020
Heterogeneity and stochastic growth regulation of biliary epithelial cells dictate dynamic epithelial tissue
Kenji Kamimoto1, Kota Kaneko1, Cindy Yuet-Yin Kok1
1Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
Abstract:
Dynamic remodeling of the intrahepatic biliary epithelial tissue plays key roles in liver regeneration, yet the cellular basis for this process remains unclear. We took an unbiased approach based on in vivo clonal labeling and tracking of biliary epithelial cells in the three-dimensional landscape, in combination with mathematical simulation, to understand their mode of proliferation in a mouse liver injury model where the nascent biliary structure formed in a tissue-intrinsic manner. An apparent heterogeneity among biliary epithelial cells was observed: whereas most of the responders that entered the cell cycle upon injury exhibited a limited and tapering growth potential, a select population continued to proliferate, making a major contribution in sustaining the biliary expansion. Our study has highlighted a unique mode of epithelial tissue dynamics, which depends not on a hierarchical system driven by fixated stem cells, but rather, on a stochastically maintained progenitor population with persistent proliferative activity.
Related Concept Videos
Liver Regeneration
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Tissue Renewal without Stem Cells
However, failure of such a system...
Cell Specific Gene Expression
Cells Coordinate Growth and Proliferation

