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Molecular identification and phylogenetical analysis of dermatophyte fungi from Latin America

Hans Garcia Garces1, Marluce F Hrycyk1, Juliana Giacobino1

  • 1Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, São Paulo, Brazil.

Mycoses
|July 20, 2016
PubMed

Insights

Molecular identification and phylogenetic analysis of dermatophytes in Latin America revealed complex fungal relationships. DNA sequencing accurately identified most fungal strains, aiding in understanding dermatophyte diversity.

Area of Science:

  • Mycology
  • Molecular Biology
  • Phylogenetics

Background:

  • Dermatophytes are keratin-degrading fungi causing human and animal infections.
  • Molecular techniques enhance identification speed, accuracy, and phylogenetic insights.
  • Understanding dermatophyte diversity is crucial for public health.

Purpose of the Study:

  • To molecularly identify dermatophytes from Brazil and Latin America.
  • To determine the phylogenetic relationships among these dermatophyte fungi.
  • To evaluate the efficacy of ribosomal DNA regions for identification and phylogeny.

Main Methods:

  • DNA extraction and PCR amplification from 45 dermatophyte strains.
  • Sequencing of nuclear ribosomal regions: ITS1-5.8S-ITS2 and D1/D2.
  • Phylogenetic analysis using the Maximum Likelihood method with MEGA 6.0 software.

Main Results:

  • Successful species-level identification of 43 out of 45 strains (95.5%) using ITS sequencing.
  • 100% identification success rate using D1/D2 sequencing.
  • Phylogenetic analyses clearly separated Trichophyton and Microsporum genera.
  • Epidermophyton floccosum showed an uncertain phylogenetic position relative to other genera, dependent on the marker used.
  • Complex interrelationships among dermatophyte fungi were elucidated.

Conclusions:

  • Both ITS and D1/D2 ribosomal regions are effective for dermatophyte identification.
  • Molecular phylogenetic analyses reveal intricate evolutionary relationships within dermatophytes.
  • This study contributes to a better understanding of dermatophyte diversity and evolution in Latin America.
Keywords:
ArthrodermataceaeDermatophytesmolecular identificationphylogeny

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