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Updated: Mar 17, 2026
A Syngeneic Murine Model of Endometriosis using Naturally Cycling Mice
Published on: November 24, 2020
Neutrophil depletion reduces endometriotic lesion formation in mice
Masashi Takamura1, Kaori Koga1, Gentaro Izumi1
1Obstetrics and Gynecology, the University of Tokyo, Tokyo, Japan.
Problem:
Neutrophils are known to be accumulated in endometriosis; however, direct evidence of the impact of neutrophils in the development of endometriosis was lacking. To clarify the importance of neutrophils, we examined the effect of neutrophil depletion on the development of endometriosis.
Method Of Study:
Ovariectomized, estradiol-replaced, 8-week-old, female BALB/c mice were injected with endometrial fragments (Day 0). Neutrophils were depleted by anti-Gr-1 antibody, either in the early stage (from Day 1 to Day 3, group E) or late stage (from Day 8 to Day 12, group L). Control mice (group C) did not receive antibodies. On Day 14, mice were killed and the number and weight of endometriotic lesions were counted and weighed.
Results:
The number of endometriotic lesions was significantly less in group E in comparison with Group C and Group L. Weight per lesion did not differ between groups.
Conclusion:
Neutrophils are essential for the initial formation of endometriosis.
