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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
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A comprehensive compilation of SUMO proteomics.

Ivo A Hendriks1,2, Alfred C O Vertegaal1

  • 1Department of Molecular Cell Biology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands.

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Summary

Small ubiquitin-like modifiers (SUMO) are crucial for cellular processes and disease. This study consolidates human SUMO proteomics data, revealing extensive protein modification with significant nuclear functions.

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Area of Science:

  • Molecular Biology
  • Proteomics
  • Genetics

Background:

  • Small ubiquitin-like modifiers (SUMO) regulate critical cellular processes.
  • SUMOylation is implicated in diseases like cancer and Alzheimer's disease.
  • Proteomic approaches have advanced the identification of sumoylated proteins and specific modification sites.

Purpose of the Study:

  • To create a comprehensive database of human SUMO proteomics data.
  • To analyze the extent and functional implications of protein sumoylation.
  • To provide insights into SUMOylation's role in nuclear functions.

Main Methods:

  • Consolidation of all available human SUMO proteomics data.
  • Bioinformatic analysis to identify sumoylated proteins and sites.
  • Clustering of sumoylated proteins into functional networks.

Main Results:

  • Identification of 3,617 proteins sumoylated at 7,327 distinct sites.
  • Demonstration that SUMOylation is a frequent protein modification.
  • Functional network analysis revealed SUMOylation's role in transcription, mRNA processing, DNA replication, and DNA-damage response.

Conclusions:

  • Protein sumoylation is a widespread post-translational modification.
  • SUMOylation plays significant roles in various nuclear processes.
  • The consolidated database serves as a valuable resource for further research into SUMOylation and related diseases.