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Published on: November 17, 2018
Magnetic Nano-Sponges for High-Capacity Protein Enrichment and Immobilization
Yuting Zhang1, Meng Yu1, Zihao Zhang1
1State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, P. R. China.
Abstract:
On the basis of the gel effect of the living RAFT (Reversible Addition-Fragmentation Chain Transfer) polymerization, magnetic nano-sponges with ultrasoft PAA net-like shells are prepared and then used for enriching the entire proteins from real biological samples with ultrahigh capacity. In addition, the immobilized enzyme Cyt c or HRP surprisingly show much higher catalytic activity than free enzymes.

