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Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
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Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

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Imaging enzyme kinetics at atomic resolution

John Spence1, Eaton Lattman2

  • 1Physics, Arizona State University , Rural Rd, Tempe, 85287-1504, USA.

Iucrj
|July 21, 2016
PubMed

Abstract:

Serial crystallography at a synchrotron has been used to obtain time-resolved atomic resolution density maps of enzyme catalysis in copper nitrite reductase. Similar XFEL studies, intended to out-run radiation damage, will also soon appear.

Keywords:
catalysisdenitrificationenzyme mechanismradiation damageradiolysisserial crystallographysynchrotron radiation

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