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Enzyme Kinetics

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...

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Related Experiment Video

Updated: Mar 17, 2026

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

Imaging enzyme kinetics at atomic resolution.

John Spence¹, Eaton Lattman²

¹Physics, Arizona State University , Rural Rd, Tempe, 85287-1504, USA.

|July 21, 2016

Summary

This summary is machine-generated.

Time-resolved serial crystallography captured atomic details of enzyme catalysis in copper nitrite reductase. Future X-ray Free Electron Laser (XFEL) studies aim to overcome radiation damage limitations.

Keywords:

catalysis denitrification enzyme mechanism radiation damage radiolysis serial crystallography synchrotron radiation

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Last Updated: Mar 17, 2026

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

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Published on: September 18, 2019

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Fixed Target Serial Data Collection at Diamond Light Source

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Published on: February 26, 2021

Area of Science:

Biochemistry
Structural Biology
Enzymology

Background:

Enzyme catalysis is fundamental to biological processes.
Understanding enzyme mechanisms at atomic resolution is crucial.
Copper nitrite reductase plays a key role in nitrogen cycling.

Purpose of the Study:

To visualize enzyme catalysis in real-time.
To elucidate the atomic details of copper nitrite reductase function.

Main Methods:

Serial crystallography using synchrotron radiation.
Time-resolved data collection.
Analysis of atomic resolution density maps.

Main Results:

Obtained time-resolved atomic resolution density maps of copper nitrite reductase.
Visualized key steps in the enzyme's catalytic cycle.

Conclusions:

Synchrotron-based serial crystallography is a powerful tool for studying enzyme dynamics.
Future X-ray Free Electron Laser (XFEL) studies will provide complementary insights, potentially mitigating radiation damage.