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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 17, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

A Simple, Versatile Antibody-Based Barcoding Method for Flow Cytometry.

Billur Akkaya¹, Pietro Miozzo², Amanda H Holstein¹

¹Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and.

Journal of Immunology (Baltimore, Md. : 1950)

|July 22, 2016

Summary

This summary is machine-generated.

This study introduces a new antibody (Ab)-based cell barcoding method for flow cytometry. This practical approach enables simultaneous analysis of pooled samples, advancing immunological research.

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Last Updated: Mar 17, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

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Published on: February 8, 2016

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Cell barcoding aids in identifying individuals within complex biological mixtures.
Current fluorescent dye-based methods limit cell barcoding's application in flow cytometry.
Flow cytometry analysis of pooled samples requires efficient cell identification methods.

Purpose of the Study:

To develop a simple and versatile cell barcoding strategy for flow cytometry.
To overcome limitations of existing fluorescent dye-based barcoding techniques.
To enable simultaneous analysis of pooled experimental samples in flow cytometry.

Main Methods:

Utilized combinations of a single antibody conjugated to different fluorochromes for barcoding.
Applied the antibody-based barcoding strategy to live and fixed/permeabilized cells.
Integrated the barcoding approach into standard flow cytometry protocols.

Main Results:

Demonstrated the efficacy of antibody-based barcoding across various experimental conditions.
Showcased the method's versatility with both live and fixed cells.
Successfully identified cells from individual samples pooled for flow cytometry analysis.

Conclusions:

Antibody-based barcoding offers a practical and simple solution for cell identification in pooled flow cytometry samples.
This method has broad applicability in immunological research.
The strategy can be integrated into diverse flow cytometry applications.