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Cell-Based Screening: Cellular Assays with a Molecular Endpoint Measured by SAMDI Mass Spectrometry (Small 28/2016)
Eric J Berns1, Maria D Cabezas1, Milan Mrksich1,2
1Department of Biomedical Engineering, Department of Chemistry, Northwestern University, Evanston, IL, 60208, USA.
Abstract:
On page 3811, M. Mrksich and co-workers culture cells using self-assembled monolayers presenting cell adhesion ligands and enzyme substrates. A lysis buffer disrupts the cell membranes, releasing enzymes that modify the immobilized substrates. These modifications can be measured with SAMDI mass spectrometry, giving a high-throughput, cell-based assay.
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