Related Experiment Video
Updated: Mar 17, 2026

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Characterization of Viral Populations by Using Circular Sequencing
Zachary J Whitfield1, Raul Andino2
1Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.
Abstract:
With the enormous sizes viral populations reach, many variants are at too low a frequency to be detected by conventional next-generation sequencing (NGS) methods. Circular sequencing (CirSeq) is a method by which the error rate of next-generation sequencing is decreased so that even low-frequency viral variants can be accurately detected. The ability to visualize almost the entire genetic makeup of a viral swarm has implications for epidemiology, viral evolution, and vaccine design. Here we discuss experimental planning, analysis, and recent insights using CirSeq.
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