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Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

Lysosomes

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...

Delivery Pathways to the Lysosome

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...

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Related Experiment Video

Updated: Mar 17, 2026

Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers Diethylaminoethyl-cellulose Columns

Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers Diethylaminoethyl-cellulose Columns

Published on: April 6, 2019

Subverting lysosomal function in Trypanosoma brucei.

¹London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, UK.

Microbial Cell (Graz, Austria)

|July 22, 2016

Summary

This summary is machine-generated.

L-leucyl-L-leucyl methyl ester (LeuLeu-OMe) shows promise as a new anti-parasitic agent against Trypanosoma brucei. This compound targets the parasite's lysosome, offering a novel strategy for disease treatment.

Keywords:

LeuLeu-OMe Trypanosoma brucei lysosome

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Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Published on: August 19, 2014

Area of Science:

Parasitology
Drug Discovery
Cell Biology

Background:

Trypanosoma brucei causes African sleeping sickness, a neglected tropical disease.
Targeting the parasite's lysosome is a viable strategy for developing new anti-parasitic drugs.

Purpose of the Study:

To evaluate the efficacy of L-leucyl-L-leucyl methyl ester (LeuLeu-OMe) as an anti-Trypanosoma brucei agent.
To explore the potential of lysosomotropic compounds in combating parasitic infections.

Main Methods:

Treatment of Trypanosoma brucei cultures with LeuLeu-OMe.
Assessment of parasite viability and lysosomal function.

Main Results:

LeuLeu-OMe demonstrated significant anti-Trypanosoma brucei activity.
The compound effectively disrupted lysosomal function in the parasite.

Conclusions:

LeuLeu-OMe is a promising candidate for the development of novel therapeutics against Trypanosoma brucei.
Subverting lysosome function represents a valuable approach for anti-parasitic drug development.