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A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
Published on: March 6, 2018
Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model
1The institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.
Abstract:
The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when subcutaneously (s.c.) grafted into syngeneic C57BL/6 host mice (Foster et al., 1997). This protocol will describe the adoptive transfer of purified CD11b+Gr1+ double positive (DP) myeloid-derived suppressor cells (MDSC) and CD3+ T cells in the TRAMP-C2 prostate cancer mouse model in order to establish the intrinsic functionality of these immune cells and to determine their role in tumorigenesis in vivo (Yan et al., 2014).
Insights
This study explores adoptive cell transfer in prostate cancer using the TRAMP-C2 mouse model. It investigates the role of myeloid-derived suppressor cells (MDSC) and T cells in tumor growth.
Area of Science:
- Immunology
- Oncology
- Cellular Biology
Background:
- Adoptive cell transfer is a promising immunotherapy for cancer, chronic infection, and autoimmunity.
- The transgenic adenocarcinoma mouse prostate (TRAMP) model and TRAMP-C2 cell line are utilized for prostate cancer research.
- TRAMP-C2 cells are tumorigenic when grafted into syngeneic mice.
Purpose of the Study:
- To describe the adoptive transfer protocol for specific immune cells in a prostate cancer model.
- To establish the intrinsic functionality of CD11b+Gr1+ double positive (DP) myeloid-derived suppressor cells (MDSC) and CD3+ T cells.
- To determine the role of these immune cells in prostate cancer tumorigenesis in vivo.
Main Methods:
- Utilizing the TRAMP-C2 prostate cancer mouse model.
- Purification of CD11b+Gr1+ double positive (DP) myeloid-derived suppressor cells (MDSC).
- Purification of CD3+ T cells for adoptive transfer.
Main Results:
- The protocol details the adoptive transfer of purified MDSC and T cells.
- The study aims to elucidate the functional capabilities of these transferred immune cells.
- Investigating the impact of these cells on prostate cancer progression in vivo.
Conclusions:
- This protocol provides a framework for studying immune cell function in prostate cancer.
- Understanding MDSC and T cell roles is crucial for developing effective immunotherapies.
- The findings will contribute to the broader field of adoptive cell transfer for cancer treatment.
