Related Experiment Video
Updated: Mar 17, 2026
Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
Published on: June 8, 2022
Neutrophil Fluorescence: A New Indicator of Cell Activation During Septic Shock-Induced Disseminated Intravascular
Laure Stiel1, Xavier Delabranche, Anne-Cécile Galoisy
11Service de Réanimation Médicale, Nouvel Hôpital Civil, Hôpitaux Universitaires de Strasbourg, Strasbourg, France. 2EA 7293, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Faculté de médecine, Université de Strasbourg, Strasbourg, France. 3Laboratoire d'hématologie et hémostase, Hôpital de Hautepierre, Hôpitaux Universitaires de Strasbourg, Strasbourg, France. 4Laboratoire de Biostatistique et d'Informatique Médicale, Faculté de Médecine, Hôpitaux Universitaires de Strasbourg, Strasbourg, France. 5Groupe Méthode en Recherche Clinique, Service de Santé Publique, Hôpitaux Universitaires de Strasbourg, Strasbourg, France. 6UMR 7213 CNRS, Faculté de Pharmacie, Université de Strasbourg, Illkirch, France. 7EA 3430, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Faculté de médecine, Université de Strasbourg, Strasbourg, France.
Objective:
To investigate the contribution of neutrophil activation as innate immune cells during septic shock-induced disseminated intravascular coagulation.
Design:
Prospective study.
Setting:
One University Hospital ICU.
Participants:
Hundred patients with septic shock. Thirty-five patients had disseminated intravascular coagulation according to Japanese Association for Acute Medicine 2006 score.
Intervention:
None.
Measurements And Main Results:
Neutrophil chromatin decondensation was assessed by measuring neutrophil fluorescence (NEUT-side-fluorescence light) labeled by a fluorochrome-based polymethine reagent using a routine automated flow cytometer Sysmex XN20 (Sysmex, Kobe, Japan) and neutrophil-derived CD66b microparticles by prothrombinase assay. Measurements in disseminated intravascular coagulation and no disseminated intravascular coagulation patients showed that a mean value of NEUT-side-fluorescence light above 57.3 arbitrary units had a sensitivity of 90.91% and a specificity of 80.60% for disseminated intravascular coagulation diagnosis. NEUT-side-fluorescence light was correlated to the CD66b microparticles/neutrophil count, a surrogate of neutrophil activation associated with septic shock-induced disseminated intravascular coagulation.
Conclusion:
NEUT-side-fluorescence light, routinely available, could prove an accurate biomarker of neutrophil activation.
