Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.5K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.5K
Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene01:13

Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene

7.6K
Bromination and chlorination of aromatic rings by electrophilic aromatic substitution reactions are easily achieved, but fluorination and iodination are difficult to achieve. Fluorine is so reactive that its reaction with benzene is difficult to control, resulting in poor yields of monofluoroaromatic products. To address this, Selectfluor reagent is used as a fluorine source in which a fluorine atom is bonded to a positively charged nitrogen.
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ambient black carbon and its dependence on in situ stubble burning.

Environmental monitoring and assessment·2026
Same author

ASPP-HAM-UNet: a modified UNet for moderate-to-severe traumatic brain injury segmentation.

Biomedical physics & engineering express·2026
Same author

Prevalence of relapse in pulmonary sarcoidosis: A systematic review and meta-analysis.

Respiratory medicine·2026
Same author

In silico and in vitro insights into stigmasterol targeting Keap1/Nrf2, Bcl-2/Bax and IKKβ/IκBα protein-protein interactions in Arsenic-induced toxicity.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same author

Correlation of Adipokines (Omentin-1 and Chemerin) in Type 2 Diabetes Mellitus with Metabolic Syndrome Patients: A Case-Control Study.

Annals of African medicine·2026
Same author

Indole-3-Acetic Acid Production and Molecular Insights into its Biosynthetic Pathway.

Indian journal of microbiology·2026

Related Experiment Video

Updated: Feb 20, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.1K

A multi writable thiophene-based selective and reversible chromogenic fluoride probe with dual -NH functionality.

Siddharth Vishwakarma1, Ajit Kumar2, Abha Pandey1

  • 1Department of Chemistry (Centre of Advanced Study), Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|July 22, 2016
PubMed
Summary
This summary is machine-generated.

A new chromogenic fluoride probe (BSB) visually detects fluoride ions (F-) with high selectivity. This probe enables the construction of a chemical INHIBIT logic gate with multi-write capabilities.

Keywords:
Chromogenic sensorDensity functional theoryFluorideLogic gatebis-Schiff base

More Related Videos

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

3.0K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.6K

Related Experiment Videos

Last Updated: Feb 20, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.1K
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

3.0K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.6K

Area of Science:

  • Chemical Sensing
  • Anion Recognition
  • Supramolecular Chemistry

Background:

  • Development of selective chemosensors for anions is crucial.
  • Fluoride ion (F-) detection is important in environmental and biological monitoring.
  • Existing probes often lack selectivity or sensitivity.

Purpose of the Study:

  • To design and synthesize a novel chromogenic probe (BSB) for selective fluoride ion detection.
  • To investigate the sensing mechanism of the probe.
  • To construct a chemical logic gate based on the probe's response.

Main Methods:

  • Synthesis and structural characterization of the BSB probe.
  • UV-visible and 1H NMR titration experiments for anion binding studies.
  • Density Functional Theory (DFT) calculations for mechanistic insights.
  • Construction and testing of a chemical INHIBIT logic gate.

Main Results:

  • The BSB probe exhibits high selectivity for F- over other anions, with a distinct color change (colorless to red).
  • Sensing mechanism involves hydrogen bonding and deprotonation between BSB and F-.
  • Limit of detection for F- is 6.9×10(-7)M, with a binding constant of 1.42±0.069×10(8)M(-2).
  • A functional INHIBIT logic gate with "Multi-write" ability was successfully constructed.

Conclusions:

  • The BSB probe is an effective tool for selective and sensitive chromogenic detection of fluoride ions.
  • The probe's response mechanism is well-understood through experimental and theoretical studies.
  • The developed chemical logic gate demonstrates potential for molecular computing applications.