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Published on: August 6, 2019
Matrix Matalloproteinase-8 Gene Polymorphism in Chronic Periapical Lesions
Background/Aim:
Increased levels of matrix metalloproteinase-8 (MMP-8) have been associated with risk of developing chronic periapical lesion (CPL). Gene polymorphisms are contributing factors in the pathogenesis of the inflammation. The aim of this study was to analyze polymorphism in the gene MMP-8 and its association and influence on clinical manifestation of CPL.
Methods:
A total of 240 unrelated Macedonian subjects were included in the present study. Polymorphism -799 C/T in the gene MMP-8 detected with restriction enzyme BgIII was studied in 120 patients with CPL and 120 controls without any signs of chronic or acute inflammatory process in the jaw. The amplification of the region of selected gene was made with polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP).
Results:
Our results showed that there were differences in the allele and genotype frequencies of the MMP8 -799 C/T polymorphisms between patients with CPL and controls (p < 0.05). Also this study suggests that MMP-8 polymorphism -799 C/T was a risk for expression of CPL (OR=8.27<3.44
Conclusion:
The detection of this genetic polymorphism is relevant for obtaining providential treatment of patients who are at high risk of chronic periapical inflammation.
Insights
Genetic variations in the matrix metalloproteinase-8 (MMP-8) gene are linked to chronic periapical lesions (CPL). The -799 C/T polymorphism in MMP-8 increases the risk of developing CPL.
Area of Science:
- Oral pathology
- Genetics
- Biochemistry
Background:
- Matrix metalloproteinase-8 (MMP-8) levels correlate with chronic periapical lesion (CPL) risk.
- Gene polymorphisms play a role in inflammatory disease pathogenesis.
Purpose of the Study:
- To investigate the association between MMP-8 gene polymorphism and CPL.
- To determine the influence of MMP-8 polymorphism on CPL clinical manifestation.
Main Methods:
- Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) used to analyze MMP-8 -799 C/T polymorphism.
- Study included 120 CPL patients and 120 controls from Macedonia.
Main Results:
- Significant differences in allele and genotype frequencies of MMP8 -799 C/T were observed between CPL patients and controls (p < 0.05).
- The MMP-8 -799 C/T polymorphism was identified as a risk factor for CPL development (OR=8.27).
Conclusions:
- The -799 C/T genetic polymorphism in the MMP-8 gene is associated with an increased risk of CPL.
- Identifying this genetic marker can aid in early detection and management of patients at high risk for chronic periapical inflammation.
