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Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Related Experiment Video

Updated: Mar 17, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

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Targeted candidate gene screens using CRISPR/Cas9 technology.

A N Shah1, C B Moens1, A C Miller2

  • 1Fred Hutchinson Cancer Research Center, Seattle, WA, United States.

Methods in Cell Biology
|July 23, 2016
PubMed
Summary
This summary is machine-generated.

CRISPR screening enables rapid functional genomics in zebrafish by efficiently mutating genes in early embryos. This powerful reverse genetics tool facilitates large-scale screening for gene function with significant advantages over traditional methods.

Keywords:
CRISPRCas9Genome editingIndelMultiplexMutationRNA-guided nucleaseReverse genetic screenZebrafishsgRNA

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Area of Science:

  • * Genomics and Proteomics
  • * Molecular Biology
  • * Developmental Biology

Background:

  • * Assigning function to the zebrafish proteome is crucial in the postgenomic era.
  • * Conventional reverse genetics methods can be time-consuming and resource-intensive.
  • * The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system offers a revolutionary approach.

Purpose of the Study:

  • * To highlight the utility of CRISPR-based screening for functional genomics in zebrafish.
  • * To demonstrate the efficiency of CRISPR technology for rapid gene mutation and phenotypic analysis.
  • * To present CRISPR screening as an accessible method for researchers with basic molecular biology skills.

Main Methods:

  • * Utilizing a two-part CRISPR system comprising a single guide RNA (sgRNA) and Cas9 enzyme.
  • * Injecting the CRISPR system into one-cell stage zebrafish embryos.
  • * Leveraging CRISPR-induced double-stranded DNA breaks to create mutations at targeted loci.

Main Results:

  • * Efficient mutation of target genes in zebrafish embryos, leading to observable phenotypes.
  • * Successful generation of phenocopies of known mutant phenotypes.
  • * Demonstration of CRISPR-based F0 screening for identifying genes involved in specific phenotypes.

Conclusions:

  • * CRISPR screening is a highly efficient and broadly applicable method for reverse genetics in zebrafish.
  • * This approach significantly accelerates the process of assigning function to zebrafish genes.
  • * CRISPR screening offers substantial benefits over conventional methods, making functional genomics more accessible.