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Activity-Dependent Palmitoylation Controls SynDIG1 Stability, Localization, and Function.

Inderpreet Kaur1, Vladimir Yarov-Yarovoy2, Lyndsey M Kirk1

  • 1Departments of Pharmacology, and.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|July 23, 2016
PubMed
Summary
This summary is machine-generated.

Synapse differentiation-induced gene I (SynDIG1) undergoes palmitoylation, a modification regulated by neuronal activity. This process is crucial for SynDIG1

Keywords:
PSD-95SynDIG1excitatory synapsepalmitoylation

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Synapses are crucial for neuronal communication.
  • Synapse differentiation-induced gene I (SynDIG1) regulates excitatory synapse composition.
  • Palmitoylation affects synaptic protein localization and function.

Purpose of the Study:

  • To investigate the role of palmitoylation in SynDIG1 stability and localization.
  • To determine how neuronal activity influences SynDIG1 palmitoylation.
  • To understand SynDIG1's contribution to synaptic function.

Main Methods:

  • Structural modeling of SynDIG1.
  • Site-directed mutagenesis to identify palmitoylation sites (C191, C192).
  • Heterologous cell expression and neuronal culture experiments.
  • Rat hippocampal slice culture to assess activity-dependent palmitoylation.

Main Results:

  • SynDIG1 is palmitoylated at cysteine residues C191 and C192.
  • Palmitoylation positively regulates SynDIG1's dendritic targeting in neurons.
  • Neuronal activity blockade increases SynDIG1 palmitoylation and synaptic localization.
  • Palmitoylation regulates SynDIG1 stability and subcellular localization.

Conclusions:

  • Palmitoylation of SynDIG1 is regulated by neuronal activity.
  • This modification is critical for SynDIG1's stability, localization, and function at synapses.
  • Activity-dependent palmitoylation of SynDIG1 influences synaptic strength.