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The effect of alkaline phosphatase on platelet aggregation
1Division of Haematology/Oncology, Roger Williams Cancer Center, Providence, Rhode Island 02908.
Haematologia
|January 1, 1989
Summary
High alkaline phosphatase levels may impair platelet aggregation, increasing bleeding risk. This study found alkaline phosphatase inhibits platelet clumping in vitro and in patients with cholestatic disease.
Area of Science:
- Biochemistry
- Hematology
- Pathophysiology
Background:
- Elevated alkaline phosphatase is associated with various diseases.
- Platelet aggregation is crucial for hemostasis.
- The direct impact of alkaline phosphatase on platelet function is not fully understood.
Purpose of the Study:
- To investigate the effect of alkaline phosphatase on platelet aggregation.
- To determine if alkaline phosphatase influences platelet response to different agonists.
- To validate in vitro findings using patient plasma.
Main Methods:
- In vitro studies using varying concentrations of alkaline phosphatase.
- Testing platelet aggregation induced by adenosine diphosphate, epinephrine, collagen, and arachidonic acid.
- Assessing ristocetin-induced platelet aggregation in a dose-dependent manner.
- Confirmation using plasma from patients with cholestatic disease.
Main Results:
- Alkaline phosphatase inhibited adenosine diphosphate-induced platelet aggregation at high concentrations.
- Similar inhibitory effects were observed with epinephrine, collagen, and arachidonic acid.
- A dose-dependent inhibition of ristocetin-induced aggregation by alkaline phosphatase was demonstrated.
- Inhibition patterns were consistent with patient plasma exhibiting elevated alkaline phosphatase.
Conclusions:
- Elevated alkaline phosphatase significantly inhibits platelet aggregation in vitro.
- Patients with markedly elevated alkaline phosphatase may exhibit impaired platelet function (thrombocytopathy).
- These findings suggest a potential link between high alkaline phosphatase and bleeding diathesis.