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Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

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Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
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Related Experiment Video

Updated: Mar 17, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

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Optical Aptasensors for Adenosine Triphosphate.

Stella Ng1, Hui Si Lim1, Qian Ma1

  • 1Department of Chemistry, National University of Singapore, Singapore 117543.

Theranostics
|July 23, 2016
PubMed
Summary

Aptamers, a type of nucleic acid, are revolutionizing biosensors for detecting adenosine triphosphate (ATP). These aptasensors offer enhanced sensitivity and selectivity for various analytes.

Keywords:
Adenosine triphosphateaptamersaptasensorschemiluminescence.colorimetryfluorometrynucleic acid

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Last Updated: Mar 17, 2026

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Area of Science:

  • Biomolecular Engineering
  • Biosensor Technology
  • Nucleic Acid Chemistry

Background:

  • Nucleic acids are versatile biomolecules with diverse structures and properties suitable for sensor applications.
  • Their biocompatibility enables their use in in vivo assays, and they are key components in various sensing devices.
  • Aptamers, specifically, have improved sensor sensitivity, selectivity, and multiplexing capabilities for diverse analytes.

Purpose of the Study:

  • To review the advancements in using aptamers as the core component in optical sensors for adenosine triphosphate (ATP) detection.
  • To highlight the sensing mechanisms, performance metrics, and applications of aptasensors for ATP.

Main Methods:

  • Review of existing literature on aptamer-based optical sensors for ATP detection.
  • Analysis of sensing mechanisms, including structural changes and signal transduction pathways.
  • Evaluation of sensor performance parameters such as sensitivity, selectivity, and detection limits.

Main Results:

  • Aptamers provide a highly specific and sensitive recognition element for ATP in optical sensing platforms.
  • Various optical detection strategies, including fluorescence, colorimetric, and electrochemical methods, have been successfully implemented.
  • Aptasensors demonstrate significant improvements in detecting ATP compared to traditional methods.

Conclusions:

  • Aptamers are highly effective recognition elements for the optical detection of ATP, offering superior performance.
  • Continued research into aptasensor design and optimization promises further advancements in ATP monitoring.
  • These aptasensors hold significant potential for applications in diagnostics, environmental monitoring, and fundamental research.