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Effects of thiabendazole on the kidneys of ICR mice
Y Tada1, M Yoneyama, J Kabashima
1Department of Toxicology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Abstract:
The effects of thiabendazole (TBZ) on the kidneys of male and female Crj:CD-1 (ICR) mice were investigated. The mice were given 0, 250 or 500 mg TBZ/kg body weight/day by gavage (using olive oil as a vehicle) for 1, 3, 5 or 7 days. The 24-hr urine volumes of treated mice were increased, significantly so in male mice given the high dose of TBZ. Protein was present in the urine of both control and treated mice throughout the experiment but electrophoresis of the protein showed the presence of a relatively high-molecular-weight protein in the urine of the treated mice. Levels of serum urea nitrogen were decreased in treated mice, but serum creatinine levels did not differ from those of controls. Relative kidney weights tended to be dose-dependently increased in comparison with the controls. Pathological examination showed that after one dose (at either level) the kidneys of males and females were swollen and white maculae were present. Microscopic examination revealed dilation of the proximal, distal and collecting tubules with flattening and degeneration of the tubular epithelium. Tubular dilation was severe in high-dose male mice. These histological changes paralleled the alterations in the 24-hr urine volume. These data suggested that the increased urine volume may be caused primarily by the prevention of reabsorption of water in the distal and collecting tubules. Electron microscopy revealed the flattening of foot processes of podocytes and oedematous changes in the mesangium of glomeruli in TBZ-treated mice. We conclude that TBZ given by gavage in olive oil affects the kidneys of ICR mice.
Insights
Thiabendazole (TBZ) administration in mice increased urine volume and affected kidney structure, indicating potential renal toxicity. These findings highlight TBZ
Area of Science:
- Nephrology
- Toxicology
- Pharmacology
Background:
- Thiabendazole (TBZ) is an anthelmintic drug.
- Understanding TBZ's renal effects is crucial for safety assessment.
Purpose of the Study:
- To investigate the effects of TBZ on mouse kidneys.
- To characterize the dose- and time-dependent renal changes induced by TBZ.
Main Methods:
- Male and female ICR mice were administered varying doses of TBZ (0, 250, 500 mg/kg/day) via gavage for 1-7 days.
- Urine volume, protein content, serum markers (urea nitrogen, creatinine), relative kidney weights, and kidney histology (light and electron microscopy) were analyzed.
Main Results:
- TBZ increased 24-hour urine volume, particularly in high-dose males, and induced high-molecular-weight proteinuria.
- Relative kidney weights increased dose-dependently, with observed kidney swelling and white maculae.
- Histopathology revealed tubular dilation, epithelial degeneration, podocyte foot process effacement, and mesangial edema, suggesting impaired water reabsorption.
Conclusions:
- TBZ administration causes significant renal alterations in mice, including tubular and glomerular damage.
- The observed increase in urine volume is likely due to impaired water reabsorption in renal tubules.
- These findings indicate that TBZ exerts nephrotoxic effects in ICR mice.