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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Reporter Genes02:11

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Related Experiment Video

Updated: Mar 17, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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RNA Fluorescence with Light-Up Aptamers.

Jonathan Ouellet1

  • 1Department of Chemistry and Physics, Monmouth University West Long Branch, NJ, USA.

Frontiers in Chemistry
|July 23, 2016
PubMed
Summary
This summary is machine-generated.

Genetically encoded biosensors using RNA aptamers and fluorogen ligands enable live-cell imaging and understanding of cellular processes. These innovative tools are evolving for diverse biological applications.

Keywords:
BroccoliMangoRNA fluorescenceSpinachgreen RNAlight-up aptamers

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • Live-cell imaging is crucial for understanding cellular signaling and physiology.
  • Green fluorescent protein (GFP) has revolutionized cellular imaging.
  • Precise control over cellular regulation necessitates advanced imaging technologies.

Purpose of the Study:

  • To review the emergence and evolution of RNA aptamer-based genetically encoded biosensors.
  • To examine the conversion of these biosensors into diverse applications.
  • To provide a global overview of RNA aptamer biosensor development.

Main Methods:

  • Review of recent literature on RNA aptamer biosensors.
  • Analysis of the mechanism of aptamer-fluorogen interactions.
  • Survey of current and potential applications in cellular research.

Main Results:

  • RNA aptamers binding fluorogen ligands represent a new class of genetically encoded biosensors.
  • These biosensors activate fluorescence upon ligand binding.
  • The field has seen rapid development and diversification of applications.

Conclusions:

  • RNA aptamer biosensors offer a powerful platform for live-cell imaging.
  • Continued evolution promises expanded utility in studying cellular functions.
  • These tools significantly advance our understanding of cellular signaling and physiology.