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Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons
Hongmei Li1, Han-A Park1, Elizabeth A Jonas2
1Department of Internal Medicine (Endocrinology), School of Medicine, Yale University, New Haven, USA.
Bio-Protocol
|July 23, 2016
Abstract:
This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in real time in cultured hippocampal neurons.
