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MALDI Imaging of Liquid Extraction Surface Analysis Sampled Tissue.

Elizabeth C Randall¹, Alan M Race¹, Helen J Cooper

¹National Physical Laboratory , Hampton Road, Teddington, Middlesex, TW11 0LW, United Kingdom.

Analytical Chemistry

|July 23, 2016

Summary

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) visualized liquid extraction surface analysis (LESA) sampling effects on mouse brain tissue. This combined approach revealed LESA

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Area of Science:

Analytical Chemistry
Biomedical Imaging
Mass Spectrometry

Background:

Combined mass spectrometry imaging (MSI) methods analyze samples using multiple techniques.
This approach allows for higher resolution examination of sampling effects and combines analytical advantages.

Purpose of the Study:

To investigate the impact of liquid extraction surface analysis (LESA) sampling on mouse brain tissue using matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI).
To understand localized tissue washing effects during LESA sampling through complementary multivariate analysis.

Main Methods:

Mouse brain tissue was analyzed using matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI).
Liquid extraction surface analysis (LESA) was employed as a sampling technique prior to MALDI MSI.
Multivariate analysis techniques, including principal component analysis (PCA), non-negative matrix factorization (NMF), and t-distributed stochastic neighbor embedding (t-SNE), were applied to the acquired MALDI MS images.

Main Results:

MALDI MS imaging successfully visualized the regions sampled by LESA.
Variability in sampling area, spatial precision, and analyte delocalization induced by LESA were assessed.
Single-ion images and multivariate analysis-derived images provided insights into LESA sampling effects.

Conclusions:

MALDI MSI is effective in visualizing LESA sampling regions on biological tissue.
Combined MSI and multivariate analysis offer a comprehensive understanding of sampling-induced alterations in tissue analysis.