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GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

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A Modular Bioplatform Based on a Versatile Supramolecular Multienzyme Complex Directly Attached to Graphene.

Abeer Alshammari¹, Mareike G Posner, Abhishek Upadhyay

¹Department of Physics, King Saud University , Riyadh 11451, Saudi Arabia.

ACS Applied Materials & Interfaces

|July 23, 2016

Summary

Researchers developed a modular bioplatform using supramolecular enzyme complexes on graphene. This adaptable graphene-based platform immobilizes enzymes without function loss, enabling versatile applications.

Keywords:

E2 complexes biosensing functional hybrid interfaces graphene supramolecular dihydrolipoyl acyltransferase complexes

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Area of Science:

Biotechnology
Materials Science
Enzyme Engineering

Background:

Developing adaptable bioplatforms is difficult due to protein immobilization challenges and the need for specific component combinations.
Protein immobilization often leads to reduced protein function, hindering the creation of effective multifunctional surfaces.

Purpose of the Study:

To introduce a generic, modular strategy for constructing adaptable and multifunctional bioplatforms.
To demonstrate the utility of supramolecular multienzyme complexes as building blocks for bioplatforms.

Main Methods:

Utilized cage-like supramolecular multienzyme complexes (Thermoplasma acidophilum dihydrolipoyl acyltransferase, E2) as adaptable building blocks.
Immobilized E2 complexes directly and noncovalently onto graphene surfaces.
Engineered multifunctionality by binding partner enzymes (E1) to the E2-graphene platform.

Main Results:

E2 complexes formed stable monolayers on graphene, preserving supramolecular structure and molecular recognition capabilities.
Immobilized enzymes (E1) retained their function, with significant catalytic activity detected over a wide substrate concentration range.
Demonstrated multiplexing capability via patterned cotransfer of modified E2 complexes.

Conclusions:

The E2-graphene system provides a robust and customizable platform for creating adaptable, multifunctional biodevices.
This modular approach overcomes key limitations in protein immobilization and enables versatile bioplatform engineering.