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Related Experiment Video

Updated: Mar 17, 2026

Standardized Colon Ascendens Stent Peritonitis in Rats - a Simple, Feasible Animal Model to Induce Septic Acute Kidney Injury
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Endothelial bioreactor system ameliorates multiple organ dysfunction in septic rats.

Shuai Ma1,2, Yuli Lin3, Bo Deng1,2

  • 1Division of Nephrology & Unit of Critical Nephrology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, 639 Zhizaoju Road, Shanghai, 200011, China.

Intensive Care Medicine Experimental
|July 23, 2016
PubMed
Summary

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The endothelial bioreactor (EBR) system improved survival rates in septic rats by modulating neutrophil activity and reducing inflammation. This extracorporeal endothelial therapy shows promise for sepsis treatment.

Area of Science:

  • Biomedical Engineering
  • Critical Care Medicine
  • Sepsis Pathophysiology

Background:

  • The endothelium plays a crucial role in sepsis, making it a potential therapeutic target.
  • Previous studies demonstrated the efficacy of an extracorporeal endothelial cell therapy system (EBR) in prolonging survival in endotoxemia sepsis models.
  • A miniature EBR system was developed for investigating therapeutic effects and mechanisms in a rat model of sepsis induced by cecal ligation and puncture (CLP).

Purpose of the Study:

  • To evaluate the therapeutic effects of a miniature endothelial bioreactor (EBR) system in a rat model of sepsis.
  • To investigate the underlying mechanisms by which the EBR system modulates sepsis progression and organ injury.
  • To assess the impact of EBR therapy on survival rates, physiological parameters, and inflammatory responses in septic rats.
Keywords:
BioreactorEndothelial cellsExtracorporeal circulationInflammationMultiple organ dysfunctionNeutrophilsSepsis

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Main Methods:

  • A miniature EBR system was utilized, involving extracorporeal circulation passing through a hemofilter and then an EBR cartridge containing endothelial cells.
  • Septic rats (CLP-induced) were treated for 4 hours with either the EBR system or a sham EBR system (without cells).
  • Physiological and biochemical parameters, cytokine levels, endothelial function, and 7-day survival were monitored. In vitro experiments assessed the effects of EBR on septic rat pulmonary endothelial cells.

Main Results:

  • The EBR system significantly increased the 7-day survival rate of septic rats from 25.0% (sham EBR) to 57.1% (p=0.04).
  • EBR therapy protected liver and renal function, reduced kidney and lung injury, and decreased pulmonary vascular leakage and inflammatory cell infiltration (especially neutrophils).
  • EBR treatment reduced pulmonary endothelial expression of intercellular adhesion molecule-1 and secretion of CXCL1/CXCL2, and modulated peripheral blood neutrophil activation (decreased CD11b expression and intracellular calcium).

Conclusions:

  • The endothelial bioreactor (EBR) system effectively ameliorated sepsis, improved survival, and preserved organ function in a rat model.
  • Extracorporeal endothelial therapy via EBR may exert its beneficial effects by modulating pulmonary endothelial cell function.
  • The EBR system appears to reduce neutrophil adhesion and chemotaxis and modulate peripheral blood neutrophil activation, contributing to its therapeutic efficacy in sepsis.