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High-throughput DNA extraction of forensic adhesive tapes
Christina Forsberg1, Linda Jansson2, Ricky Ansell3
1Swedish National Forensic Centre, Linköping, Sweden.
Forensic Science International. Genetics
|July 23, 2016
Summary
A new direct lysis DNA extraction protocol for forensic tape-lifting samples offers higher yields and amplifiability. This efficient method reduces manual labor and increases throughput for casework analysis.
Area of Science:
- Forensic Science
- Molecular Biology
- Biotechnology
Background:
- Tape-lifting is a common forensic DNA sampling method.
- DNA extraction from tape is challenging due to sample properties.
Purpose of the Study:
- Develop and validate a direct lysis DNA extraction protocol for adhesive tapes.
- Improve efficiency and yield for forensic casework.
Main Methods:
- Developed a direct lysis protocol using Chelex beads and SceneSafe FAST tape.
- Validated the method through in-house testing and casework implementation.
Main Results:
- The direct lysis protocol yielded higher mean DNA amounts compared to PrepFiler Express BTA.
- Casework implementation showed an increase in DNA concentrations above 0.01ng/μL from 71% to 76%.
- Reduced manual labor by 50% and doubled laboratory throughput for tape samples.
Conclusions:
- The direct lysis protocol is efficient, reproducible, and suitable for forensic casework.
- This simplified method provides a cost-effective alternative for high-throughput DNA extraction from challenging samples.
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